Description
Deep transcriptome sequencing (RNA-seq) provides massive and valuable information about functional elements in the genome.
Using RNA-seq data, people can profile gene expression change, interrogate alternative splicing events, uncover novel transcribed regions, detect aberrant transcripts (such as gene fusions) and coding variants, etc.
Ideally, transcriptome sequencing should be able to directly identify and quantify all RNA species, small or large, low or high abundance.
However, RNA-seq is a complicated, multistep process involving sample preparation, amplification, fragmentation, purification and sequencing. A single improper operation would result in biased or even unusable data.
It is always a good practice to check the quality of your RNA-seq data before pursuing any directions listed above.
EVER-seq is a free and open-source package (Evaluate Experiment of RNA-seq) to comprehensively quality control RNA-seq datasets.
EVER-seq is cross-platform and it works on Mac OS X, Windows and Linux.
Detailed instructions on how to install and use the EVER-seq utility on your Mac are available HERE.
User Reviews for EVER-seq FOR MAC 1
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for EVER-seq FOR MAC
EVER-seq FOR MAC is a fantastic tool for quality controlling RNA-seq data. Its cross-platform compatibility makes it a must-have for researchers.