Description
Double-stranded DNA is able to form triple-helical structures by accommodating a third nucleotide strand in its major groove. This sequence-specific process offers a potent mechanism for targeting genomic loci of interest, that is of great interest for bio-technological and gene-therapeutic applications.
Triplex-formation requires a duplex, presumably DNA, and a single-stranded nucleotide sequence acting as the third strand, presumably RNA.
The process of predicting putative triplexes in sequence data can be divided into three steps:
· identifying the triplex-forming oligonucleotides (TFO) in single-stranded sequences
· identifying the triplex target sites in double-stranded sequences able to accomondate a third strand
· assessing the compatibility of potential TFO/TTS pairs according to the canonical triplex formation rules
Triplexator is an application that uses approximate pattern matching based on the triplex formation rules in order to find putative TFO-TTS pairs (triplexes) in two sets of nucleotide sequences, where one set contains the duplex sequences and the other th
Detailed instructions on how to install and use the Triplexator utility on your Mac are available HERE.
User Reviews for Triplexator FOR MAC 1
-
for Triplexator FOR MAC
Triplexator FOR MAC is a powerful tool for predicting triplexes in DNA sequences. The installation instructions are clear and helpful.